Aspects of Nucleic Acid Composition in Gossypium

Nucleic Acid Metabolism during Germination of Pima Cotton (Gossypium barbadense).

The nucleic acid metabolism in long staple Pima cotton (Gossypium barbadense L.) was examined during the first 48 hours of germination at 34 C. Specific activity analysis of pulselabeled nucleic acids indicated that the first significant incorporation of precursors into DNA occurs at approximately 18 hours when the radicles are emerging with a peak of incorporation occurring at 36 hours. Specif...

Nucleic Acid Hybridization – General Aspects

Fundamental aspects of the nucleic acid i-motif structures

The i-motif structure is formed in cytosine-rich sequences, being its building block the cytosine•cytosine+ base pair. This structure is particularly stable at pH values below the physiological one (~7.4) and, because of that, it has not attracted as much biological interest as other non-canonical structures such as the G-quadruplex. Nowadays, the proposal of potential roles in vivo, as well as...

Nucleic Acid Composition of Bone Marrow Mononuclear Cells

Recent studies using anion exclusion chromatography have suggested that uracil is misincorporated into the DNA of patients with megaloblastic anemia to levels detectable by nonradioactive methods. We have investigated the nucleotide composition of DNA from the bone marrow mononuclear cells of eight patients with cobalamin deficiency and compared this with that found in normal subjects. The medi...

The composition of uterine and endometrial nucleic acid.

It has been shown by Vendrely and Vendrely (1, 2) that the average desoxypentose nucleic acid (DNA) content per cell is constant in several tissues. Employing microspectrophotometric analysis of single cells, Leuchtenberger and Schrader (3) have pointed out that the cellular DNA content of the same type of cell may vary by as much as a factor of 30 and that the DNA content of each cell is relat...